How many cells are counted in a central counting area?

If you count over only 5 of the 25 large squares, then multiply that value by 5 to obtain the number of cells per central counting area. At least two chambers should be counted, including at least 100 cells within each central counting area of each chamber.
For More Information Please Refer:
http://www.vivo.colostate.edu/hbooks/pathphys/reprod/semeneval/hemacytometer.html
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